PLANT REPRODUCTIVE

Plant reproduction is the process by which plants generate new individuals, or offspring. Reproduction is either sexual or asexual. Sexual reproduction is the formation of offspring by the fusion of gametes . Asexual reproduction is the formation of offspring without the fusion of gametes. Sexual reproduction results in offspring genetically different from the parents. Asexual offspring are genetically identical except for mutation. In higher plants, offspring are packaged in a protective seed, which can be long lived and can disperse the offspring some distance from the parents. In flowering plants (angiosperms), the seed itself is contained inside a fruit, which may protect the developing seeds and aid in their dispersal.

Sexual Reproduction in Angiosperms: Ovule Formation

All plants have a life cycle that consists of two distinct forms that differ in size and the number of chromosomes per cell. In flowering plants, the

A hibiscus flower, showing anthers, five stigmas, and pollen.

large, familiar form that consists of roots, shoots, leaves, and reproductive structures (flowers and fruit) is diploid and is called the sporophyte. The sporophyte produces haploid microscopic gametophytes that are dependent on tissues produced by the flower. The reproductive cycle of a flowering plant is the regular, usually seasonal, cycling back and forth from sporophyte to gametophyte. The flower produces two kinds of gametophytes, male and female. The female gametophyte arises from a cell within the ovule , a small structure within the ovary of the flower. The ovary is a larger structure within the flower that contains and protects usually many ovules. Flowering plants are unique in that their ovules are entirely enclosed in the ovary. The ovary itself is part of a larger structure called the carpel, which consists of the stigma, style, and ovary. Each ovule is attached to ovary tissue by a stalk called the funicle. The point of attachment of the funicle to the ovary is called the placenta.
As the flower develops from a bud, a cell within an ovule called the archespore enlarges to form an embryo-sac mother cell (EMC). The EMC divides by meiosis to produce four megaspores. In this process the number of chromosomes is reduced from two sets in the EMC to one set in the megaspores, making the megaspores haploid. Three of the four megaspores degenerate and disappear, while the fourth divides mitotically three times to produce eight haploid cells. These cells together constitute the female gametophyte, called the embryo sac.
The eight embryo sac cells differentiate into two synergids, three antipodal cells, two fused endosperm nuclei, and an egg cell. The mature embryo sac is situated at the outer opening (micropyle) of the ovule, ready to receive the sperm cells delivered by the male gametophyte.

Pollen

The male gametophyte is the mature pollen grain. Pollen is produced in the anthers, which are attached at the distal end of filaments. The filament and anther together constitute the stamen, the male sex organ. Flowers usually produce many stamens just inside of the petals. As the flower matures, cells in the anther divide mitotically to produce pollen mother cells (PMC). The PMCs divide by meiosis to produce haploid microspores in groups of four called tetrads. The microspores are housed within a single layer of cells called the tapetum, which provides nutrition to the developing pollen grains.
Each microspore develops a hard, opaque outer layer called the exine, which is constructed from a lipoprotein called sporopollenin. The exine has characteristic pores, ridges, or projections that can often be used to identify a species, even in fossil pollen. The microspore divides mitotically once or twice to produce two or three haploid nuclei inside the mature pollen grain. Two of the nuclei function as sperm nuclei that can eventually fuse with the egg and endosperm nuclei of the embryo sac, producing an embryo and endosperm, respectively.
For sexual fusion to take place, however, the pollen grain must be transported to the stigma, which is a receptive platform on the top of the style, an elongated extension on top of the carpel(s). Here the moist surface or chemicals cause the pollen grain to germinate. Germination is the growth of a tube from the surface of a pollen grain. The tube is a sheath of pectin , inside of which is a solution of water, solutes , and the two or three nuclei, which lack any cell walls. Proper growth of the pollen tube requires an aqueous solution of appropriate solute concentration, as well as nutrients such as boron, which may aid in its synthesis of pectin.
At the apex of the tube are active ribosomes and endoplasmic reticulum (types of cell organelles ) involved in protein synthesis. Pectinase and a glucanase (both enzymes that break down carbohydrates ) probably maintain flexibility of the growing tube and aid in penetration. The pollen tube apex also releases ribonucleic acid (RNA) and ribosomes into the tissues of the style. The tube grows to eventually reach the ovary, where it may travel along intercellular spaces until it reaches a placenta. Through chemical recognition, the pollen tube changes its direction of growth and penetrates through the placenta to the ovule. Here the tube reaches the embryo sac lying close to the micropyle, and sexual fertilization takes place.

Double Fertilization

Fertilization in flowering plants is unique among all known organisms, in that not one but two cells are fertilized, in a process called double fertilization. One sperm nucleus in the pollen tube fuses with the egg cell in the embryo sac, and the other sperm nucleus fuses with the diploid endosperm nucleus. The fertilized egg cell is a zygote that develops into the diploid embryo of the sporophyte. The fertilized endosperm nucleus develops into the triploid endosperm, a nutritive tissue that sustains the embryo and seedling. The only other known plant group exhibiting double fertilization is the Gnetales in the genus Ephedra, a nonflowering seed plant. However, in this case the second fertilization product degenerates and does not develop into endosperm.
Double fertilization begins when the pollen tube grows into one of the two synergid cells in the embryo sac, possibly as a result of chemical attraction to calcium. After penetrating the synergid, the apex of the pollen tube breaks open, releasing the two sperm nuclei and other contents into the synergid. As the synergid degenerates, it envelops the egg and endosperm cells, holding the two sperm nuclei close and the other expelled contents of the pollen tube. The egg cell then opens and engulfs the sperm cell, whose membrane breaks apart and allows the nucleus to move near the egg nucleus. The nuclear envelopes then disintegrate, and the two nuclei combine to form the single diploid nucleus of the zygote. The other sperm cell fuses with the two endosperm nuclei, forming a single triploid cell, the primary endosperm cell, which divides mitotically into the endosperm tissue.
Double fertilization and the production of endosperm may have contributed to the great ecological success of flowering plants by accelerating the growth of seedlings and improving survival at this vulnerable stage. Faster seedling development may have given flowering plants the upper hand in competition with gymnosperm seedlings in some habitats, leading to the abundance of flowering plants in most temperate and tropical regions. Gymnosperms nevertheless are still dominant at higher elevations and latitudes, and at low elevations in the Pacific Northwest coniferous forests, such as the coastal redwoods. The reasons for these patterns are still controversial.

Read more: http://www.biologyreference.com/Re-Se/Reproduction-in-Plants.html#ixzz43sM6xcD0

source:http://www.biologyreference.com/Re-Se/Reproduction-in-Plants.htmlhttp://www.biologyreference.com/Re-Se/Reproduction-in-Plants.html

Rules Netiquette

The etiquette guidelines that govern behavior when communicating on the Internet have become known as netiquette. Netiquette covers not only rules of behavior during discussions but also guidelines that reflect the unique electronic nature of the medium. Netiquette usually is enforced by fellow users who are quick to point out infractions of netiquette rules. The summary of email rules in the information below is based on published sources such as Shea's (2004) online book, Netiquette.

• Identify yourself:
  • Begin messages with a salutation and end them with your name.
  • Use a signature (a footer with your identifying information) at the end of a message
• Include a subject line. Give a descriptive phrase in the subject line of the message header that tells the topic of the message (not just "Hi, there!").
• Avoid sarcasm. People who don't know you may misinterpret its meaning.
• Respect others' privacy. Do not quote or forward personal email without the original author's permission.
• Acknowledge and return messages promptly.
• Copy with caution. Don't copy everyone you know on each message.
• No spam (a.k.a. junk mail). Don't contribute to worthless information on the Internet by sending or responding to mass postings of chain letters, rumors, etc.
• Be concise. Keep messages concise—about one screen, as a rule of thumb.
• Use appropriate language:
  • Avoid coarse, rough, or rude language.
  • Observe good grammar and spelling.
• Use appropriate emoticons (emotion icons) to help convey meaning. Use "smiley's" or punctuation such as :-) to convey emotions. See website list of emoticons at http://netlingo.com/smiley.cfm and http://www.robelle.com/smugbook/smiley.html.
• Use appropriate intensifiers to help convey meaning.
  • Avoid "flaming" (online "screaming") or sentences typed in all caps.
  • Use asterisks surrounding words to indicate italics used for emphasis (*at last*).
  • Use words in brackets, such as (grin), to show a state of mind.
  • Use common acronyms (e.g., LOL for "laugh out loud"). 
  
  source: http://www.education.com/reference/article/netiquette-rules-behavior-internet/http://www.education.com/reference/article/netiquette-rules-behavior-internet/
  
  
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CHEMISTRY IN MY BRAIN

BUFFER SOLUTION 
 

figure1 buffer solution

 What is buffer solution?
solution that has ability to maintain its pH when small amount of strong acid or strong base is added to the solution.

*Calculating buffer pH

Monoprotic acids

First write down the equilibrium expression.

HA A⁻ + H⁺

This shows that when the acid dissociates equal amounts of hydrogen ion and anion are produced. The equilibrium concentrations of these three components can be calculated in an ICE table.

ICE table for a monoprotic acid

	[HA]	[A−]	[H+]
I	C0	0	y
C	-x	x	x
E	C0-x	x	x+y

The first row, labelled I, lists the initial conditions: the concentration of acid is C₀, initially undissociated, so the concentrations of A⁻ and H⁺ would be zero; y is the initial concentration of added strong acid, such as hydrochloric acid. If strong alkali, such as sodium hydroxide, is added y will have a negative sign because alkali removes hydrogen ions from the solution. The second row, labelled C for change, specifies the changes that occur when the acid dissociates. The acid concentration decreases by an amount -x and the concentrations of A⁻ and H⁺ both increase by an amount +x. This follows from the equilibrium expression. The third row, labelled E for equilibrium concentrations, adds together the first two rows and shows the concentrations at equilibrium.
To find x, use the formula for the equilibrium constant in terms of concentrations:

Substitute the concentrations with the values found in the last row of the ICE table:

Simplify to:

With specific values for C₀, K_a and y this equation can be solved for x. Assuming that pH = -log₁₀[H⁺] the pH can be calculated as pH = -log₁₀(x+y).

source: https://en.wikipedia.org/wiki/Buffer_solution#Calculating_buffer_pHhttps://en.wikipedia.org/wiki/Buffer_solution#Calculating_buffer_pH

RECOMBINANT DNA

BIOLOGY

The Basics of Recombinant DNA

figure1:Step making DNA

So What Is rDNA?
That's a very good question! rDNA stands for recombinant DNA. Before
we get to the "r" part, we need to understand DNA. Those of you with
a background in biology probably know about DNA, but a lot of ChemE's haven't
seen DNA since high school biology. DNA is the keeper of the all the information
needed to recreate an organism. All DNA is made up of a base consisting
of sugar, phosphate and one nitrogen base. There are four nitrogen bases,
adenine (A), thymine (T), guanine (G), and cytosine (C). The nitrogen
bases are found in pairs, with A & T and G & C paired together. The sequence

of the nitrogen bases can be arranged in an infinite ways, and their structure is known as

the famous "double helix" which  is shown in the image below. The sugar used in

DNA is deoxyribose. The four nitrogen bases are the same for all organisms. The

sequence and number of bases is what creates diversity.  DNA does not

actually make the organism, it only makes  proteins. The DNA is transcribed

into mRNA and mRNA is translated into protein, and the protein  then forms the

organism. By changing  the DNA sequence, the way in which the  protein is

formed changes. This leads to either a different protein, or an inactive protein
 
How is Recombinant DNA made?
There are three different methods by which Recombinant DNA is made. They are
Transformation, Phage Introduction, and Non-Bacterial Transformation. Each
are described separately below.

  Why is rDNA important?
Recombinant DNA has been gaining in importance over the last few years, and
recombinant DNA will only become more important in the 21st century as genetic

diseases become more prevelant and agricultural area is reduced.  Below  are

some of the areas where Recombinant DNA will have an impact.

• Better Crops (drought & heat resistance)
• Recombinant Vaccines (ie. Hepatitis B)
• Prevention and cure of sickle cell anemia
• Prevention and cure of cystic fibrosis
• Production of clotting factors
• Production of insulin
• Production of recombinant pharmaceuticals
• Plants that produce their own insecticides
• Germ line and somatic gene therapy 

source:https://www.blogger.com/blogger.g?blogID=822989321202057055#editor/target=post;postID=3948061916385342144https://www.blogger.com/blogger.g?blogID=822989321202057055#editor/target=post;postID=3948061916385342144